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Polihexanid, Antisepsis, Antiseptik, Wundinfektion, Wunde <Motiv>, Octenidindihydrochlorid, Polihexanid, Polyhexamethylenbiguanid, Prüfkörpertest, Wundantiseptik, octenidine dihydrochloride, polihexanide, polyhexamethylene biguanide, disc carrier test, wound antisepsis Background: The antimicrobial efficacy of an octenidine-wound gel in lower than the commercially available concentrations (0.005 % - 0.1 %) should be determined. Because lower concentrations show a lower cytotoxicity, the benefit of octenidine in the treatment of chronic wounds can be estimated by this investigation. For comparison, a wound gel with 0.02 % polihexanide was tested. Material and methods: A practical disc carrier test (phase 2/step 2) was established for this study. The used test organisms were S. aureus, S. epidermidis, E. faecium, P. aeruginosa, S. marcescens and C. albicans. The tests were performed without challenge and with MEM + 10 % FBS, simulating wound fluid. Exposure times between 5 min - 24 h were checked. Results and conclusion: The practical test demonstrated that higher concentrations of antiseptics and longer exposure times are required than those determined in the quantitative suspension test. Octenidine 0.02 % reached (with wound fluid simulation) after 24 h exposure time a sufficient antiseptic efficacy (> 3 lg-reduction) against all tested organisms. Octenidine 0.02 % obviously exceeded polihexanide 0.02 % in efficacy (no sufficient reduction of C. albicans, E. faecium). With the concentration of 0.02 % the tissue compatibility increased significantly. Therefore, products with octenidine 0.02 % could be a reliable alternative for the treatment of chronic wounds in future.
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